what is the relative convenience of pour and streak plate technique?
Q. In culturing clinical specimens?
Asked by ..:::DeliciousDyme:::.. - Sun Apr 12 18:00:01 2009 - - 1 Answers - 0 Comments
A. Yes, in culturing clinical specimens.
Answered by Plucky - Sun Apr 12 21:29:58 2009
Q. In culturing clinical specimens?
Asked by ..:::DeliciousDyme:::.. - Sun Apr 12 18:00:01 2009 - - 1 Answers - 0 Comments
A. Yes, in culturing clinical specimens.
Answered by Plucky - Sun Apr 12 21:29:58 2009
Which of the following counting techniques doesn't differentiate between live and dead bacterial cells?
Q. Which of the following counting techniques doesn't differentiate between live and dead bacterial cells in a culture? A)serial dilution B)spread plate C)pour plate D)direct microscopic count
Asked by Sam L - Fri Apr 24 16:52:19 2009 - - 2 Answers - 0 Comments
A. D)direct microscopic count
Answered by niaji - Fri Apr 24 17:43:19 2009
Q. Which of the following counting techniques doesn't differentiate between live and dead bacterial cells in a culture? A)serial dilution B)spread plate C)pour plate D)direct microscopic count
Asked by Sam L - Fri Apr 24 16:52:19 2009 - - 2 Answers - 0 Comments
A. D)direct microscopic count
Answered by niaji - Fri Apr 24 17:43:19 2009
1. What are the principles of the various sterilization methods?
Q. 2. What is the relative merits of the pour-plate method as compared with streak-plate method for the isolation of bacteria? 3. What are methods that I can use to obtain a pure culture and what's the significance? 4. What are the differences between lawn culture and pour plate techniques?
Asked by ee von - Wed May 30 21:23:55 2007 - - 5 Answers - 0 Comments
A. 1. Autoclaving materials (intense heat and pressure) should sterilize any media or glassware. 10% bleach , then 70% EtOH is used to wipe counterspace. Flame sterilization can occur with prior dipping in 95%EtOH (spreader) or not (loop). If you are in a hood, you can also sterilize with UV light. In all these situations, the intense heat/chemical conditions will denature proteins/disrupt chemical bonds. Flaming the opening of a (glass) bottle will create an updraft and prevent microbes in the air from falling into the bottle. 2. With a pour plate, you don't need to prep plates ahead of time and it's easier to visualize/measure how many microbes per volume or mass sample. 3. To obtain a pure culture, grow a lawn and select desired colony.… [cont.]
Answered by joie_du_cor - Wed May 30 21:49:23 2007
Q. 2. What is the relative merits of the pour-plate method as compared with streak-plate method for the isolation of bacteria? 3. What are methods that I can use to obtain a pure culture and what's the significance? 4. What are the differences between lawn culture and pour plate techniques?
Asked by ee von - Wed May 30 21:23:55 2007 - - 5 Answers - 0 Comments
A. 1. Autoclaving materials (intense heat and pressure) should sterilize any media or glassware. 10% bleach , then 70% EtOH is used to wipe counterspace. Flame sterilization can occur with prior dipping in 95%EtOH (spreader) or not (loop). If you are in a hood, you can also sterilize with UV light. In all these situations, the intense heat/chemical conditions will denature proteins/disrupt chemical bonds. Flaming the opening of a (glass) bottle will create an updraft and prevent microbes in the air from falling into the bottle. 2. With a pour plate, you don't need to prep plates ahead of time and it's easier to visualize/measure how many microbes per volume or mass sample. 3. To obtain a pure culture, grow a lawn and select desired colony.… [cont.]
Answered by joie_du_cor - Wed May 30 21:49:23 2007
plz help got exam!!!?
Q. A turbid broth culture of Escherichia coli was enumerated using a counting chamber (haemocytometer) and gave an average of 5.2 bacterial cells per square ( of volume 2.5 x 10-7 cm3 ). A viable count was carried out on the same culture using a pour plate method, incorporating 1cm3 samples of a range of dilutions into agar plates. The average of triplicate samples was 206 colonies per plate of the 10-4 dilution. Calculate the total count of the E.coli culture and suggest a possible reason for the difference between the two colony counts.
Asked by simran - Wed Dec 26 15:44:18 2007 - - 2 Answers - 0 Comments
A. This is a question to ask in the physics forum. Someone with that expertise should be able to handle this problem with ease.
Answered by JasonM - Sun Dec 30 15:41:29 2007
Q. A turbid broth culture of Escherichia coli was enumerated using a counting chamber (haemocytometer) and gave an average of 5.2 bacterial cells per square ( of volume 2.5 x 10-7 cm3 ). A viable count was carried out on the same culture using a pour plate method, incorporating 1cm3 samples of a range of dilutions into agar plates. The average of triplicate samples was 206 colonies per plate of the 10-4 dilution. Calculate the total count of the E.coli culture and suggest a possible reason for the difference between the two colony counts.
Asked by simran - Wed Dec 26 15:44:18 2007 - - 2 Answers - 0 Comments
A. This is a question to ask in the physics forum. Someone with that expertise should be able to handle this problem with ease.
Answered by JasonM - Sun Dec 30 15:41:29 2007
dilution problem..microbiology?
Q. You wish to determine viable counts on a culture of Bacillus subtilis . You begin by pipetting 1 ml of culture into 99 ml of sterile water. After shaking the dilution well you make a series of 2 further dilutions but of 10-1 each. From the most dilute you make three pour plates using 1 ml in each. After incubation you find the plate counts are 182, 167 and 175. What is the viable count (cells/ml) in the original culture? why is 182 used and not the other 2 values?
Asked by sweety p - Sun Mar 22 17:38:07 2009 - - 3 Answers - 0 Comments
A. Pipetting 1 mL into 99 mL of water is a 100 times dilution, or 10^2. Adding two 10^1 dilutions brings the total dilution to 10^4. The average of 182, 167 and 175 is about 175 (actually 174.6). 174.6 x 10^4 = 1.745 x 10^6, or 1,745,000 cells/mL. Hope that helps.
Answered by cdela12345 - Sun Mar 22 18:20:50 2009
Q. You wish to determine viable counts on a culture of Bacillus subtilis . You begin by pipetting 1 ml of culture into 99 ml of sterile water. After shaking the dilution well you make a series of 2 further dilutions but of 10-1 each. From the most dilute you make three pour plates using 1 ml in each. After incubation you find the plate counts are 182, 167 and 175. What is the viable count (cells/ml) in the original culture? why is 182 used and not the other 2 values?
Asked by sweety p - Sun Mar 22 17:38:07 2009 - - 3 Answers - 0 Comments
A. Pipetting 1 mL into 99 mL of water is a 100 times dilution, or 10^2. Adding two 10^1 dilutions brings the total dilution to 10^4. The average of 182, 167 and 175 is about 175 (actually 174.6). 174.6 x 10^4 = 1.745 x 10^6, or 1,745,000 cells/mL. Hope that helps.
Answered by cdela12345 - Sun Mar 22 18:20:50 2009
The Culture of Organism?
Q. How do the colonies on the surface of the pour plate differ from those suspended in the agar?
Asked by jericholic85 - Tue Mar 4 12:50:03 2008 - - 1 Answers - 0 Comments
A. In the pour plate method, the colonies grow embedded in the agar and so you can count the individual isolated colonies to calculate the CFU (colony forming unit). However in the suspended agar, you can just see if there is growth, check oxygen requirements, but colonies will not be isolated.
Answered by i bloo - Tue Mar 4 14:32:47 2008
Q. How do the colonies on the surface of the pour plate differ from those suspended in the agar?
Asked by jericholic85 - Tue Mar 4 12:50:03 2008 - - 1 Answers - 0 Comments
A. In the pour plate method, the colonies grow embedded in the agar and so you can count the individual isolated colonies to calculate the CFU (colony forming unit). However in the suspended agar, you can just see if there is growth, check oxygen requirements, but colonies will not be isolated.
Answered by i bloo - Tue Mar 4 14:32:47 2008
Can anyone check my french paper and make any neccessary changes?
Q. Avoir une longue ligne de culture et de diversite raffinee, Madagascar a devenu un des les nations plus uniques dans le monde. Madagascar est une isle dans le pais d'Afrique. Il se trouve dans l ocean Indien au sud-est d Afrique. Il y a encore des choses a Madagascar qu on ne trouve dans autres endroits. On peut trouver une tres bonne culture, histoire, et des choses incroyables a Madagascar. Madagascar est la quatrieme plus grande ile au monde. La ile de Madagascar est divise en cing regions qui sont Les Montagnes Centrales, la Cote des Iles Vierges, La Cote des Contrastes, La Cote du Palissandre, et La Cote de Capricorne. La plus grande ville de Madagascar est Antananarivo qui est aussi le capital. Au Madagascar vous pouvez trouver… [cont.]
Asked by FishyFace - Wed Dec 12 23:31:45 2007 - - 2 Answers - 0 Comments
A. Avec une longue ligne de culture et de diversite raffinee, le Madagascar est devenu l une des nations plus uniques du monde. Le Madagascar est une ile par le continent de l'Afrique. Il se trouve dans l ocean indien au sud-est d Afrique. Il y a encore des choses au Madagascar que l on ne trouve dans aucuns autres endroits. On peut y trouver une culture et une histoire qui sont toutes tres belles ; il y a aussi d autres choses incroyables au Madagascar. Le Madagascar est la quatrieme plus grande ile au monde. L ile de Madagascar est divisee en cinq regions dont les Montagnes Centrales, la Cote des Iles Vierges, la Cote des Contrastes, la Cote du Palissandre, et la Cote de Capricorne. La plus grande ville du Madagascar est Antananarivo qui… [cont.]
Answered by mguardian_north - Fri Dec 14 18:10:24 2007
Q. Avoir une longue ligne de culture et de diversite raffinee, Madagascar a devenu un des les nations plus uniques dans le monde. Madagascar est une isle dans le pais d'Afrique. Il se trouve dans l ocean Indien au sud-est d Afrique. Il y a encore des choses a Madagascar qu on ne trouve dans autres endroits. On peut trouver une tres bonne culture, histoire, et des choses incroyables a Madagascar. Madagascar est la quatrieme plus grande ile au monde. La ile de Madagascar est divise en cing regions qui sont Les Montagnes Centrales, la Cote des Iles Vierges, La Cote des Contrastes, La Cote du Palissandre, et La Cote de Capricorne. La plus grande ville de Madagascar est Antananarivo qui est aussi le capital. Au Madagascar vous pouvez trouver… [cont.]
Asked by FishyFace - Wed Dec 12 23:31:45 2007 - - 2 Answers - 0 Comments
A. Avec une longue ligne de culture et de diversite raffinee, le Madagascar est devenu l une des nations plus uniques du monde. Le Madagascar est une ile par le continent de l'Afrique. Il se trouve dans l ocean indien au sud-est d Afrique. Il y a encore des choses au Madagascar que l on ne trouve dans aucuns autres endroits. On peut y trouver une culture et une histoire qui sont toutes tres belles ; il y a aussi d autres choses incroyables au Madagascar. Le Madagascar est la quatrieme plus grande ile au monde. L ile de Madagascar est divisee en cinq regions dont les Montagnes Centrales, la Cote des Iles Vierges, la Cote des Contrastes, la Cote du Palissandre, et la Cote de Capricorne. La plus grande ville du Madagascar est Antananarivo qui… [cont.]
Answered by mguardian_north - Fri Dec 14 18:10:24 2007
What are the signs of growths in a liquid medium?
Q. The most common growth media for microorganisms are nutrient broths (liquid nutrient medium), liquid media are often mixed with agar and poured into petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured. Bacteria grown in liquid cultures often form colloidal suspensions. (need help with my homework in Microbiology) *Added Questions-please do answer these two: -why is culture tube should be flamed when opened and closed? (usually done inside the laboratory) - why is culture tube should be held horizontally? *I really can't fine the answer on the net that's why I posted it here*
Asked by ChocoCrumble - Sun Jun 29 01:39:46 2008 - - 1 Answers - 0 Comments
A. You flame it to kill off any possible unwanted micro-organisms residing around the opening that could fall in. You don't want them in there. Holding the tube horizontally, or at least not vertical, is also to prevent unwanted micro-organisms from falling in.
Answered by theezeefje2002 - Tue Jul 1 19:44:40 2008
Q. The most common growth media for microorganisms are nutrient broths (liquid nutrient medium), liquid media are often mixed with agar and poured into petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured. Bacteria grown in liquid cultures often form colloidal suspensions. (need help with my homework in Microbiology) *Added Questions-please do answer these two: -why is culture tube should be flamed when opened and closed? (usually done inside the laboratory) - why is culture tube should be held horizontally? *I really can't fine the answer on the net that's why I posted it here*
Asked by ChocoCrumble - Sun Jun 29 01:39:46 2008 - - 1 Answers - 0 Comments
A. You flame it to kill off any possible unwanted micro-organisms residing around the opening that could fall in. You don't want them in there. Holding the tube horizontally, or at least not vertical, is also to prevent unwanted micro-organisms from falling in.
Answered by theezeefje2002 - Tue Jul 1 19:44:40 2008
plz help got exam?
Q. A turbid broth culture of Escherichia coli was enumerated using a counting chamber (haemocytometer) and gave an average of 5.2 bacterial cells per square ( of volume 2.5 x 10-7 cm3 ). A viable count was carried out on the same culture using a pour plate method, incorporating 1cm3 samples of a range of dilutions into agar plates. The average of triplicate samples was 206 colonies per plate of the 10-4 dilution. Calculate the total count of the E.coli culture and suggest a possible reason for the difference between the two colony counts.
Asked by simran - Sat Dec 29 21:28:38 2007 - - 1 Answers - 0 Comments
A. From the hemocytometer you have 5.2 cells per 2.5E-7 cm^3. This works out to 2.08E7 cells per cm^3. From the plate count you have 206 colonies after 4 10-fold serial dilutions, so that's 2.06E6 cells per cm^3. This means there is a 10-fold difference between what is found with the hemocytometer and the plate methods, 10x more found with the hemocytometer. Possible sources of the discrepancy: The hemocytometer counts cells in the solution, the plate count is counting cells that are able to form colonies, in other words it is measuring viability. It is possible that only 10% of the cells in the solution are viable. Another possibility is that the serial dilutions were done incorrectly. This would be a terrible reason for the counts… [cont.]
Answered by biochemist - Sat Dec 29 23:37:00 2007
Q. A turbid broth culture of Escherichia coli was enumerated using a counting chamber (haemocytometer) and gave an average of 5.2 bacterial cells per square ( of volume 2.5 x 10-7 cm3 ). A viable count was carried out on the same culture using a pour plate method, incorporating 1cm3 samples of a range of dilutions into agar plates. The average of triplicate samples was 206 colonies per plate of the 10-4 dilution. Calculate the total count of the E.coli culture and suggest a possible reason for the difference between the two colony counts.
Asked by simran - Sat Dec 29 21:28:38 2007 - - 1 Answers - 0 Comments
A. From the hemocytometer you have 5.2 cells per 2.5E-7 cm^3. This works out to 2.08E7 cells per cm^3. From the plate count you have 206 colonies after 4 10-fold serial dilutions, so that's 2.06E6 cells per cm^3. This means there is a 10-fold difference between what is found with the hemocytometer and the plate methods, 10x more found with the hemocytometer. Possible sources of the discrepancy: The hemocytometer counts cells in the solution, the plate count is counting cells that are able to form colonies, in other words it is measuring viability. It is possible that only 10% of the cells in the solution are viable. Another possibility is that the serial dilutions were done incorrectly. This would be a terrible reason for the counts… [cont.]
Answered by biochemist - Sat Dec 29 23:37:00 2007
From Yahoo Answer Search: 'POUR PLATE cultures'
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