Supposed to perform Gram stain on a sample from a pure culture of bacteria and observed a field of red and?
Q. Supposed to perform Gram stain on a sample from a pure culture of bacteria and observed a field of red and purple cocci. Adjacent cells were not always the same color. What is the conclusion?
Asked by pia - Sat Feb 16 00:38:31 2008 - - 1 Answers - 1 Comments
A. Because you said that this is a pure culture, you can conclude that they are gram-positive cocci. That's the whole answer, and you're done here. I've included more if you're interested for real life cases where you don't know if it's a pure culture or a contaminated one. If you're not interested, skip the rest of this. ---Extra discussion of real-Life problems a purple organism is definitely a gram-positive coccus. The commonest of these are streptococcus (including pneumococcus) and staphylococcus. Gram-negative cocci are orangish-red, and include organisms like gonococcus and meningococcus. The red cocci that you sometimes see may be gram-negative cocci or poorly stained gram positives. The next two photos will illustrate the… [cont.]
Answered by Yaybob - Sat Feb 16 01:43:02 2008
Q. Supposed to perform Gram stain on a sample from a pure culture of bacteria and observed a field of red and purple cocci. Adjacent cells were not always the same color. What is the conclusion?
Asked by pia - Sat Feb 16 00:38:31 2008 - - 1 Answers - 1 Comments
A. Because you said that this is a pure culture, you can conclude that they are gram-positive cocci. That's the whole answer, and you're done here. I've included more if you're interested for real life cases where you don't know if it's a pure culture or a contaminated one. If you're not interested, skip the rest of this. ---Extra discussion of real-Life problems a purple organism is definitely a gram-positive coccus. The commonest of these are streptococcus (including pneumococcus) and staphylococcus. Gram-negative cocci are orangish-red, and include organisms like gonococcus and meningococcus. The red cocci that you sometimes see may be gram-negative cocci or poorly stained gram positives. The next two photos will illustrate the… [cont.]
Answered by Yaybob - Sat Feb 16 01:43:02 2008
Why is the counter stain for the gram stain procedure different from the counter stain used for the acid-fast?
Q. Help please Why is the counter stain for the gram stain procedure different from the counter stain used for the acid-fast stain procedure? This is the last question in need to answer on my lab report thats due tomorrow morning, help if you can! Thanks
Asked by iDriveAlude - Mon Mar 10 00:41:40 2008 - - 2 Answers - 0 Comments
A. The counter stain is different cause the main stain is different...gram stain is purple...and we don't usually counter stain it in a smear..just a tissue section would get a counter stain to help see the organism...so eosin is pink and would give good contrast TB is an acid fast organism...that won't stain with the gram stain...its also a slow grower and very small... the ZN stain is used at my work...If I remember correctly...its pink or pinky red...and so you cannot have a pink counter stain... In the Hand E stain the counter stain is the eosin..which is pink...so you would need a colour that enhanced the part which you are looking at... Actually the best one is the flourescent stains...easier to see...but I think it has a high… [cont.]
Answered by ptolomy - Mon Mar 10 00:57:36 2008
Q. Help please Why is the counter stain for the gram stain procedure different from the counter stain used for the acid-fast stain procedure? This is the last question in need to answer on my lab report thats due tomorrow morning, help if you can! Thanks
Asked by iDriveAlude - Mon Mar 10 00:41:40 2008 - - 2 Answers - 0 Comments
A. The counter stain is different cause the main stain is different...gram stain is purple...and we don't usually counter stain it in a smear..just a tissue section would get a counter stain to help see the organism...so eosin is pink and would give good contrast TB is an acid fast organism...that won't stain with the gram stain...its also a slow grower and very small... the ZN stain is used at my work...If I remember correctly...its pink or pinky red...and so you cannot have a pink counter stain... In the Hand E stain the counter stain is the eosin..which is pink...so you would need a colour that enhanced the part which you are looking at... Actually the best one is the flourescent stains...easier to see...but I think it has a high… [cont.]
Answered by ptolomy - Mon Mar 10 00:57:36 2008
Why do gram positive and gram negative bacteria stain differently with the Gram stain?
Q. Why do gram positive and gram negative bacteria stain differently with the Gram stain?
Asked by on_gt - Mon Mar 19 08:41:01 2007 - - 2 Answers - 0 Comments
A. "Gram-positive bacteria are those that retain a crystal violet dye during the Gram stain process. Gram-positive bacteria appear blue or violet under a microscope, while Gram-negative bacteria appear red or pink. The Gram classification system is empirical, and largely based on differences in cell wall structure" This, and more specific information is available on Wikipedia, the free, online encyclopedia at:
Answered by cfpops - Mon Mar 19 08:44:56 2007
Q. Why do gram positive and gram negative bacteria stain differently with the Gram stain?
Asked by on_gt - Mon Mar 19 08:41:01 2007 - - 2 Answers - 0 Comments
A. "Gram-positive bacteria are those that retain a crystal violet dye during the Gram stain process. Gram-positive bacteria appear blue or violet under a microscope, while Gram-negative bacteria appear red or pink. The Gram classification system is empirical, and largely based on differences in cell wall structure" This, and more specific information is available on Wikipedia, the free, online encyclopedia at:
Answered by cfpops - Mon Mar 19 08:44:56 2007
What color do you expect to see if you do a Gram stain on an endospore-producing bacterium?
Q. What color do you expect to see if you do a Gram stain on an endospore-producing bacterium? Choose one answer. a. green b. red c. purple d. blue ok so im not sure but i think i've narrowed it down to purple or red. But im not sure.
Asked by caseychimento - Sun Feb 1 15:56:58 2009 - - 4 Answers - 0 Comments
A. If they are rods (bacillus), then they are gram positive, endospore-forming rods, and the answer would be violet (purple), answer c.
Answered by N E - Sun Feb 1 16:33:30 2009
Q. What color do you expect to see if you do a Gram stain on an endospore-producing bacterium? Choose one answer. a. green b. red c. purple d. blue ok so im not sure but i think i've narrowed it down to purple or red. But im not sure.
Asked by caseychimento - Sun Feb 1 15:56:58 2009 - - 4 Answers - 0 Comments
A. If they are rods (bacillus), then they are gram positive, endospore-forming rods, and the answer would be violet (purple), answer c.
Answered by N E - Sun Feb 1 16:33:30 2009
bacterial colony morphology and Gram stain images?
Q. I am working on a report for my Microbiology class, and I am looking for some good images of agar plates with colonies and Gram stain images of Lactobacillus acidophilus, Citrobacter koseri, Acinetobacter calcoaceticus and Bacillus sphaericus. My lab atlas doesn't have images of these specific organisms. Any suggestions of reliable sites where I can get these images? Thanks.
Asked by zoey723 - Tue Dec 22 11:50:02 2009 - - 1 Answers - 0 Comments
A. Google images is very good at looking for a wide variety of images, includes scientific ones. Just type the name of the organism you're looking for in the search bar and you should find some good pictures of them.
Answered by Eric B - Tue Dec 22 15:34:37 2009
Q. I am working on a report for my Microbiology class, and I am looking for some good images of agar plates with colonies and Gram stain images of Lactobacillus acidophilus, Citrobacter koseri, Acinetobacter calcoaceticus and Bacillus sphaericus. My lab atlas doesn't have images of these specific organisms. Any suggestions of reliable sites where I can get these images? Thanks.
Asked by zoey723 - Tue Dec 22 11:50:02 2009 - - 1 Answers - 0 Comments
A. Google images is very good at looking for a wide variety of images, includes scientific ones. Just type the name of the organism you're looking for in the search bar and you should find some good pictures of them.
Answered by Eric B - Tue Dec 22 15:34:37 2009
what characteristics are predicted by whether a bacterium retains the gram stain?
Q. what characteristics are predicted by whether a bacterium retains the gram stain?
Asked by Visis - Wed May 27 15:20:25 2009 - - 3 Answers - 0 Comments
A. There are PLENTY of pathogenic bacteria that are gram-positive (i.e. MRSA, strep, tetanus, listeria) AND gram-negative (Salmonella, E. coli, plague, meningococcal meningitis, gonorrhea). Gram-positive bacteria hold onto the purple stain when subject to an acid-alcohol decolorizer, whereas gram-negative bacteria lose the purple stain and thus pick up the pink counterstain after decolorization. Gram-positive bacteria are more susceptible to beta-lactam antibiotics (like penicillin), and gram-positive rods (Bacillus sp. and Clostridium sp.) are the only bacteria that form endospores. Also, gram-positives are almost always susceptible to vancomycin (except for vancomycin-resistant enterococci and a few others). Gram-positive bacteria will be… [cont.]
Answered by Purrmew - Wed May 27 15:43:50 2009
Q. what characteristics are predicted by whether a bacterium retains the gram stain?
Asked by Visis - Wed May 27 15:20:25 2009 - - 3 Answers - 0 Comments
A. There are PLENTY of pathogenic bacteria that are gram-positive (i.e. MRSA, strep, tetanus, listeria) AND gram-negative (Salmonella, E. coli, plague, meningococcal meningitis, gonorrhea). Gram-positive bacteria hold onto the purple stain when subject to an acid-alcohol decolorizer, whereas gram-negative bacteria lose the purple stain and thus pick up the pink counterstain after decolorization. Gram-positive bacteria are more susceptible to beta-lactam antibiotics (like penicillin), and gram-positive rods (Bacillus sp. and Clostridium sp.) are the only bacteria that form endospores. Also, gram-positives are almost always susceptible to vancomycin (except for vancomycin-resistant enterococci and a few others). Gram-positive bacteria will be… [cont.]
Answered by Purrmew - Wed May 27 15:43:50 2009
Assume you have performed a Gram stain on a sample of pus from a patient s urethra. ?
Q. Assume you have performed a Gram stain on a sample of pus from a patient s urethra. You see red, nucleated cells( >10 um) and purple rods (2.5 um). What can you conclude?
Asked by StudiousN20 - Sun Oct 26 20:35:53 2008 - - 4 Answers - 0 Comments
A. gram stain is a red herring. patient probably has chlamydia. This is clearly NOT gonorrhea - because gonorrhea is a gram-negative diplococcus. the gram positive rods are normal skin commensals - probably corynebacterium, and should be ignored. The fact that your neutrophils are stained red means that the slide WAS made properly, and decolorized properly, so I think we know that these rods are NOT gram negative. You also need to know that there is NO gram - positive rod that would cause a syndrome of urethritis. If the patient complains of urinary burning, and has this gram stain, you should be thinking of chlamydia -- a pathogen which cannot be seen on the gram stain -- the rods in this case are a red herring. The value of a gram… [cont.]
Answered by germ guy - Mon Oct 27 04:40:20 2008
Q. Assume you have performed a Gram stain on a sample of pus from a patient s urethra. You see red, nucleated cells( >10 um) and purple rods (2.5 um). What can you conclude?
Asked by StudiousN20 - Sun Oct 26 20:35:53 2008 - - 4 Answers - 0 Comments
A. gram stain is a red herring. patient probably has chlamydia. This is clearly NOT gonorrhea - because gonorrhea is a gram-negative diplococcus. the gram positive rods are normal skin commensals - probably corynebacterium, and should be ignored. The fact that your neutrophils are stained red means that the slide WAS made properly, and decolorized properly, so I think we know that these rods are NOT gram negative. You also need to know that there is NO gram - positive rod that would cause a syndrome of urethritis. If the patient complains of urinary burning, and has this gram stain, you should be thinking of chlamydia -- a pathogen which cannot be seen on the gram stain -- the rods in this case are a red herring. The value of a gram… [cont.]
Answered by germ guy - Mon Oct 27 04:40:20 2008
what part of the bacterial cell in most involved with Gram stain, and why?
Q. what part of the bacterial cell in most involved with Gram stain, and why? answer email to me!!
Asked by veniesh - Fri Sep 12 21:09:02 2008 - - 3 Answers - 0 Comments
Q. what part of the bacterial cell in most involved with Gram stain, and why? answer email to me!!
Asked by veniesh - Fri Sep 12 21:09:02 2008 - - 3 Answers - 0 Comments
My gram stain resulted in GRAM POSITIVE COCCI IN PAIRS-occassional/ single-occassional.What disease do i have?
Q. Pus cells where 14-18/HPF. My doctor said i have chlamydia but I already took a gram of azythromycin recommended by another doctor before I had the gram stain about 2 months ago.
Asked by coconutkilla - Thu Jul 10 00:26:05 2008 - - 1 Answers - 0 Comments
Q. Pus cells where 14-18/HPF. My doctor said i have chlamydia but I already took a gram of azythromycin recommended by another doctor before I had the gram stain about 2 months ago.
Asked by coconutkilla - Thu Jul 10 00:26:05 2008 - - 1 Answers - 0 Comments
The appearance of gran negative bacteria after addition of the decolorizing agent in the Gram Stain?
Q. The appearance of gran negative bacteria after addition of the decolorizing agent in the Gram Stain. a. Purple b. Red c. Colorless d. Brown e. None of the above **Please explain answer**
Asked by Allie30 - Sat Sep 5 00:54:36 2009 - - 1 Answers - 0 Comments
A. Red, Gram negative bacteria does not have a high amount of peptidoglycan in their cell wall which the purple stain binds to. Since it doesn't stain purple, it stains red by the counterstain.
Answered by Ryan - Sat Sep 5 01:29:06 2009
Q. The appearance of gran negative bacteria after addition of the decolorizing agent in the Gram Stain. a. Purple b. Red c. Colorless d. Brown e. None of the above **Please explain answer**
Asked by Allie30 - Sat Sep 5 00:54:36 2009 - - 1 Answers - 0 Comments
A. Red, Gram negative bacteria does not have a high amount of peptidoglycan in their cell wall which the purple stain binds to. Since it doesn't stain purple, it stains red by the counterstain.
Answered by Ryan - Sat Sep 5 01:29:06 2009
gram stain can find the result for clamaydia.that is true?
Q. i have a friend she's wonder why to her vagina discharge..and she came the doctor told her to gram stain her vagina to know if she have a clamaydia..that is true to find the result for clamaydia,tru gram stain?
Asked by chatterly petter - Wed Feb 6 01:27:24 2008 - - 4 Answers - 0 Comments
A. A gram stain will show inflammatory cells which might suggest an infection by chlamydia or other bacteria, but you cannot specifically identify chlamydia as the cause of the inflammation on a gram stain alone. Chlamydia bacteria are very small - only 0.25 micron in diameter, and are usually hard to see under a light microcope. The bacteria that cause gonorrhoea, on the other hand, are about twice the diameter, and can usually be seen on a gram stain as gram negative intracellular diploccoci.
Answered by __A_YAHOO_USER__ - Wed Feb 6 04:17:13 2008
Q. i have a friend she's wonder why to her vagina discharge..and she came the doctor told her to gram stain her vagina to know if she have a clamaydia..that is true to find the result for clamaydia,tru gram stain?
Asked by chatterly petter - Wed Feb 6 01:27:24 2008 - - 4 Answers - 0 Comments
A. A gram stain will show inflammatory cells which might suggest an infection by chlamydia or other bacteria, but you cannot specifically identify chlamydia as the cause of the inflammation on a gram stain alone. Chlamydia bacteria are very small - only 0.25 micron in diameter, and are usually hard to see under a light microcope. The bacteria that cause gonorrhoea, on the other hand, are about twice the diameter, and can usually be seen on a gram stain as gram negative intracellular diploccoci.
Answered by __A_YAHOO_USER__ - Wed Feb 6 04:17:13 2008
do i get accurate result if i used 10% alcohol to decolorize my gram stain?
Q. i was reading online and it says i have to used 95% alcohol for gram stain... is that one of the reason why my organism appear purple even though its negative!! because nothing grew on my MSA plate (positive organisms grow on it) but when i did my gran stain i was seeing gram positive!! and endospores
Asked by jhen - Fri Jul 31 01:25:14 2009 - - 1 Answers - 0 Comments
A. You usually don't see endospores on a gram stained slide. But, you are not using enough alcohol on the slide. Hold the slide at a 45 degree angle, and slowly add alcohol drops to it and you should see some color which is usually purple sliding down the slide and as so as the color stops sliding along with the alcohol, you should stop adding it. The best thing to do is to do 2 gram stains on the same organism just to make sure you are right. Maybe nothing grew on your MSA plate because the loop was too hot and you killed all the bacteria, or your technique is wrong. Always do your tests in twos.
Answered by Amal - Fri Jul 31 03:27:47 2009
Q. i was reading online and it says i have to used 95% alcohol for gram stain... is that one of the reason why my organism appear purple even though its negative!! because nothing grew on my MSA plate (positive organisms grow on it) but when i did my gran stain i was seeing gram positive!! and endospores
Asked by jhen - Fri Jul 31 01:25:14 2009 - - 1 Answers - 0 Comments
A. You usually don't see endospores on a gram stained slide. But, you are not using enough alcohol on the slide. Hold the slide at a 45 degree angle, and slowly add alcohol drops to it and you should see some color which is usually purple sliding down the slide and as so as the color stops sliding along with the alcohol, you should stop adding it. The best thing to do is to do 2 gram stains on the same organism just to make sure you are right. Maybe nothing grew on your MSA plate because the loop was too hot and you killed all the bacteria, or your technique is wrong. Always do your tests in twos.
Answered by Amal - Fri Jul 31 03:27:47 2009
Why is it important to perform a gram stain on a sample before culturing it on selective media?
Q. Why is it important to perform a gram stain on a sample before culturing it on selective media?
Asked by Katie - Fri Feb 5 20:58:54 2010 - - 1 Answers - 0 Comments
A. You need to select your selective media. Morphology and staining characteristics can direct you in a matter of minutes.
Answered by novangelis - Fri Feb 5 21:06:11 2010
Q. Why is it important to perform a gram stain on a sample before culturing it on selective media?
Asked by Katie - Fri Feb 5 20:58:54 2010 - - 1 Answers - 0 Comments
A. You need to select your selective media. Morphology and staining characteristics can direct you in a matter of minutes.
Answered by novangelis - Fri Feb 5 21:06:11 2010
How does Gram stain work, and how is it useful to doctors treating bacterial diseases?
Q. How does Gram stain work, and how is it useful to doctors treating bacterial diseases?
Asked by candy - Fri Mar 20 12:15:18 2009 - - 2 Answers - 0 Comments
A. The Gram stain is one of the first and most important means of identifying bacteria. It works by staining the bacteria with a dye, usually crystal violet, and this stains all bacteria (whether Gram positive or Gram negative) purple. The next step is to use iodine on the bacteria for about 1 minute and this will act to fix the dye onto the bacteria. Next the slide the bacteria is one is flooded with a solution of alcohol and acetone for 10-30 seconds. What this does is all of the dye in the Gram negative cell walls have been washed away by the alcohol, acetone, or even 95% ethanol can be used. This is because the gram negative cell wall, while it does have a layer of peptidoglycan it is significantly thinner than gram positive bacteria.… [cont.]
Answered by satts8243 - Fri Mar 20 13:04:27 2009
Q. How does Gram stain work, and how is it useful to doctors treating bacterial diseases?
Asked by candy - Fri Mar 20 12:15:18 2009 - - 2 Answers - 0 Comments
A. The Gram stain is one of the first and most important means of identifying bacteria. It works by staining the bacteria with a dye, usually crystal violet, and this stains all bacteria (whether Gram positive or Gram negative) purple. The next step is to use iodine on the bacteria for about 1 minute and this will act to fix the dye onto the bacteria. Next the slide the bacteria is one is flooded with a solution of alcohol and acetone for 10-30 seconds. What this does is all of the dye in the Gram negative cell walls have been washed away by the alcohol, acetone, or even 95% ethanol can be used. This is because the gram negative cell wall, while it does have a layer of peptidoglycan it is significantly thinner than gram positive bacteria.… [cont.]
Answered by satts8243 - Fri Mar 20 13:04:27 2009
How do you know if you have done a Gram stain properly?
Q. This is a microbiology extra point question. the instructor said it's so easy we'll not believe it. It's so easy i don't know it though!!! I may be overlooking the obvious.
Asked by ersatz_angel - Thu Jun 12 11:47:26 2008 - - 5 Answers - 0 Comments
A. If you have pink rods and purple cocci, you have stained correctly. If you have pink rods and cocci you have over-decolorized. If you have purple rods and cocci, you have under-decolorized.
Answered by Sci~Teacher - Thu Jun 12 12:15:15 2008
Q. This is a microbiology extra point question. the instructor said it's so easy we'll not believe it. It's so easy i don't know it though!!! I may be overlooking the obvious.
Asked by ersatz_angel - Thu Jun 12 11:47:26 2008 - - 5 Answers - 0 Comments
A. If you have pink rods and purple cocci, you have stained correctly. If you have pink rods and cocci you have over-decolorized. If you have purple rods and cocci, you have under-decolorized.
Answered by Sci~Teacher - Thu Jun 12 12:15:15 2008
What part of the cell are we staining using the Gram stain?
Q. My idea is that it can be the cell walls but I am not 100% sure HELP!!!
Asked by Super Girl - Wed Feb 17 02:04:19 2010 - - 2 Answers - 0 Comments
A. Super girl, your intuition wins this round. Gram staining can tell us what kind of cell wall your organism has (gram positive or gram negative). More specifically it can tell you the composition of that cell wall. Gram positive: If your cell is gram positive you can expect a purple stain. Gram positive cell walls contain a large amount of peptidoglycan. Gram negative: If your cell is gram negative, it will stain pink. Gram negative cell walls are composed of a smaller amount of peptidoglycan. Those are just the obvious differences. There are a few more that you can deduce now that you know the composition of the cell walls. Best of luck to you and your education!
Answered by pinata knife surprise - Wed Feb 17 02:13:11 2010
Q. My idea is that it can be the cell walls but I am not 100% sure HELP!!!
Asked by Super Girl - Wed Feb 17 02:04:19 2010 - - 2 Answers - 0 Comments
A. Super girl, your intuition wins this round. Gram staining can tell us what kind of cell wall your organism has (gram positive or gram negative). More specifically it can tell you the composition of that cell wall. Gram positive: If your cell is gram positive you can expect a purple stain. Gram positive cell walls contain a large amount of peptidoglycan. Gram negative: If your cell is gram negative, it will stain pink. Gram negative cell walls are composed of a smaller amount of peptidoglycan. Those are just the obvious differences. There are a few more that you can deduce now that you know the composition of the cell walls. Best of luck to you and your education!
Answered by pinata knife surprise - Wed Feb 17 02:13:11 2010
Why do Gram + and Gram - cells stain differently?
Q. Gram + stains with a blue or violet color, and gram - stain with a pink or red tint. Why is this? Thanks.
Asked by Whitney - Mon Aug 31 09:58:29 2009 - - 3 Answers - 0 Comments
A. Gram's iodine chemically links crystal violet to the peptidoglycan cell wall. Gram positive bacteria have an abundant cell wall, thus take up large amounts of stain. Gram negative bacteria have a thin cell wall (so the small amount of blue they take up is minor compared to red counterstain (safronin).
Answered by novangelis - Mon Aug 31 10:41:33 2009
Q. Gram + stains with a blue or violet color, and gram - stain with a pink or red tint. Why is this? Thanks.
Asked by Whitney - Mon Aug 31 09:58:29 2009 - - 3 Answers - 0 Comments
A. Gram's iodine chemically links crystal violet to the peptidoglycan cell wall. Gram positive bacteria have an abundant cell wall, thus take up large amounts of stain. Gram negative bacteria have a thin cell wall (so the small amount of blue they take up is minor compared to red counterstain (safronin).
Answered by novangelis - Mon Aug 31 10:41:33 2009
How do you explain the chemical basis of gram stain technique?
Q. How do you explain the chemical basis of gram stain technique?
Asked by dvyg - Thu Apr 24 10:31:43 2008 - - 2 Answers - 0 Comments
A. as above, the gram stain differentiates between two classes of microbe the gram positive have a thick peptidaglycan rich cell wall the gram negative have two membranes and very little peptidaglycan the Gram stain is a key test in identifying microbes, there are a few others you can carry out, and finaly do an API test.
Answered by homebrewbeermonster - Thu Apr 24 11:29:05 2008
Q. How do you explain the chemical basis of gram stain technique?
Asked by dvyg - Thu Apr 24 10:31:43 2008 - - 2 Answers - 0 Comments
A. as above, the gram stain differentiates between two classes of microbe the gram positive have a thick peptidaglycan rich cell wall the gram negative have two membranes and very little peptidaglycan the Gram stain is a key test in identifying microbes, there are a few others you can carry out, and finaly do an API test.
Answered by homebrewbeermonster - Thu Apr 24 11:29:05 2008
Why is the gram stain of both practical and theoretical importance in microbiology?
Q. Why is the gram stain of both practical and theoretical importance in microbiology?
Asked by jabin - Tue Aug 11 17:28:45 2009 - - 3 Answers - 0 Comments
Q. Why is the gram stain of both practical and theoretical importance in microbiology?
Asked by jabin - Tue Aug 11 17:28:45 2009 - - 3 Answers - 0 Comments
What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
Q. What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
Asked by Jamie K - Wed Sep 23 13:30:23 2009 - - 1 Answers - 0 Comments
A. Genera such as Actinomyces, Arthobacter, Corynebacterium, Mycobacterium, and Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining of these Gram-positive cells. Cultures of Bacillus, Butyrivibrio, and Clostridium a decrease in peptidoglycan thickness during growth coincides with an increase in the number of cells that stain Gram-negative. In addition, in all bacteria stained using the Gram stain, the age of the culture may influence the results of the stain. Hope this information was of help.
Answered by Strange Myth - Thu Sep 24 10:23:46 2009
Q. What are two reasons why gram-positive cells may appear pink after the completion of the gram-stain?
Asked by Jamie K - Wed Sep 23 13:30:23 2009 - - 1 Answers - 0 Comments
A. Genera such as Actinomyces, Arthobacter, Corynebacterium, Mycobacterium, and Propionibacterium have cell walls particularly sensitive to breakage during cell division, resulting in Gram-negative staining of these Gram-positive cells. Cultures of Bacillus, Butyrivibrio, and Clostridium a decrease in peptidoglycan thickness during growth coincides with an increase in the number of cells that stain Gram-negative. In addition, in all bacteria stained using the Gram stain, the age of the culture may influence the results of the stain. Hope this information was of help.
Answered by Strange Myth - Thu Sep 24 10:23:46 2009
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