Why we use a well isolated colony for the preparation of a pure culture?
Q. Why we use a well isolated colony for the preparation of a pure culture?
Asked by Pinky - Thu Dec 6 11:43:48 2007 - - 2 Answers - 0 Comments
A. Theoretically, each colony on your dish comes from a single bacteria, and therefore have the exact same genetic makeup. You'd need a "well isolated" colony because you wouldn't want other surrounding colonies to contaminate the colony you want to pick.
Answered by john d - Thu Dec 6 12:10:17 2007
Q. Why we use a well isolated colony for the preparation of a pure culture?
Asked by Pinky - Thu Dec 6 11:43:48 2007 - - 2 Answers - 0 Comments
A. Theoretically, each colony on your dish comes from a single bacteria, and therefore have the exact same genetic makeup. You'd need a "well isolated" colony because you wouldn't want other surrounding colonies to contaminate the colony you want to pick.
Answered by john d - Thu Dec 6 12:10:17 2007
T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Q. T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Asked by sweetness - Sun Apr 19 19:20:16 2009 - - 1 Answers - 0 Comments
A. Generally, we work on the assumption that an isolated colony is pure based on the appearance of surrounding colonies. If they are too dense, then do a dilution of the original sample or of a selected colony and streak again. Presumed pure colonies should be separated by half an inch or more. Traditionally, the 'proof' is in the successive reculturing of a colony, seeing that it breeds true, and in microscopic examination, looking for differently shaped bacteria, etc. There are so many biochemical tests that can be performed and so many bacteria that are similar in appearance and biochemistry that none of these methods are foolproof. If you are looking for a conclusive proof, I suppose one could use genome sequencing if it were a known,… [cont.]
Answered by hogwaump - Thu Apr 23 11:29:27 2009
Q. T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Asked by sweetness - Sun Apr 19 19:20:16 2009 - - 1 Answers - 0 Comments
A. Generally, we work on the assumption that an isolated colony is pure based on the appearance of surrounding colonies. If they are too dense, then do a dilution of the original sample or of a selected colony and streak again. Presumed pure colonies should be separated by half an inch or more. Traditionally, the 'proof' is in the successive reculturing of a colony, seeing that it breeds true, and in microscopic examination, looking for differently shaped bacteria, etc. There are so many biochemical tests that can be performed and so many bacteria that are similar in appearance and biochemistry that none of these methods are foolproof. If you are looking for a conclusive proof, I suppose one could use genome sequencing if it were a known,… [cont.]
Answered by hogwaump - Thu Apr 23 11:29:27 2009
What explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Q. What explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Asked by sassy050205 - Tue Mar 18 14:23:34 2008 - - 2 Answers - 0 Comments
A. Maybe the substance you're trying to isolate was grown on the incorrect plate (some substances grow on saline plates, other needs a broad plate like McConkey agar). Or maybe they only grow at certain temps, or also only in the dark or light. Or maybe the agar was bad, or there was poor streaking technique.
Answered by I Penguins - Tue Mar 18 14:48:22 2008
Q. What explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Asked by sassy050205 - Tue Mar 18 14:23:34 2008 - - 2 Answers - 0 Comments
A. Maybe the substance you're trying to isolate was grown on the incorrect plate (some substances grow on saline plates, other needs a broad plate like McConkey agar). Or maybe they only grow at certain temps, or also only in the dark or light. Or maybe the agar was bad, or there was poor streaking technique.
Answered by I Penguins - Tue Mar 18 14:48:22 2008
Why is it necessary to have well isolated colonies of bacteria when conducting an experiment?
Q. Why is it necessary to have well isolated colonies of bacteria when conducting an experiment?
Asked by Alaina M - Mon Oct 19 19:47:11 2009 - - 1 Answers - 0 Comments
A. If you're conducting a laboratory experiment regarding a particular species of bacteria, you want to make sure that your colonies are well isolated. The reasons for this are so that: 1) you can be sure you're dealing only with your bacterial species and have no cross contamination, and 2) so that you're isolating only one clonal organism. Most colonies on a plate started from a single bacterium, and therefore the colony you see is made up of bacteria that are all genetically identical (AKA clonal). When conducting experiments, you want to be dealing with only one strain, not a mixture of different strains... which is why you go for the isolated colony.
Answered by Vicki - Wed Oct 21 11:24:38 2009
Q. Why is it necessary to have well isolated colonies of bacteria when conducting an experiment?
Asked by Alaina M - Mon Oct 19 19:47:11 2009 - - 1 Answers - 0 Comments
A. If you're conducting a laboratory experiment regarding a particular species of bacteria, you want to make sure that your colonies are well isolated. The reasons for this are so that: 1) you can be sure you're dealing only with your bacterial species and have no cross contamination, and 2) so that you're isolating only one clonal organism. Most colonies on a plate started from a single bacterium, and therefore the colony you see is made up of bacteria that are all genetically identical (AKA clonal). When conducting experiments, you want to be dealing with only one strain, not a mixture of different strains... which is why you go for the isolated colony.
Answered by Vicki - Wed Oct 21 11:24:38 2009
How can I prepare a bread sample to successfully observe isolated bacterial colonies on agar plates?
Q. I mean how can the bread be prepared? Thanks for any help.
Asked by Jen - Tue Mar 20 17:37:23 2007 - - 1 Answers - 0 Comments
A. not to avoid your question but there is a computer simulation program called "life" that very accurately models the behavior of bacterial colonies. some versions even include the concept of two or more competing colonies. 's_Game_of_Life
Answered by michaell - Tue Mar 20 18:03:55 2007
Q. I mean how can the bread be prepared? Thanks for any help.
Asked by Jen - Tue Mar 20 17:37:23 2007 - - 1 Answers - 0 Comments
A. not to avoid your question but there is a computer simulation program called "life" that very accurately models the behavior of bacterial colonies. some versions even include the concept of two or more competing colonies. 's_Game_of_Life
Answered by michaell - Tue Mar 20 18:03:55 2007
what explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Q. what explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Asked by richie - Wed Feb 6 22:30:11 2008 - - 1 Answers - 0 Comments
A. There are a few explanations. The first is that the antibiotics are bad, which caused every cell to grow (both selected for and non selected for). This only holds true if you're using a plate with antibiotics. For example, if you have an LB-AMP plate, and you streaked with cells that were AMP resistant, but the AMP was bad, you would still get cells to grow that were not AMP resistant. Another problem would be with the experimenter themselves. To do a streak plate, you must streak out some (about a quarter of the plate), then flame the streaker. Then you cool the streaker by sticking it in some unused corner of the plate. Then you take the now sterile streaker and streak from one line from the last set of streaks. You repeat this four… [cont.]
Answered by Jay Jay - Wed Feb 6 22:37:56 2008
Q. what explanation could be given for the failure of obtaining isolated colonies on a streak plate?
Asked by richie - Wed Feb 6 22:30:11 2008 - - 1 Answers - 0 Comments
A. There are a few explanations. The first is that the antibiotics are bad, which caused every cell to grow (both selected for and non selected for). This only holds true if you're using a plate with antibiotics. For example, if you have an LB-AMP plate, and you streaked with cells that were AMP resistant, but the AMP was bad, you would still get cells to grow that were not AMP resistant. Another problem would be with the experimenter themselves. To do a streak plate, you must streak out some (about a quarter of the plate), then flame the streaker. Then you cool the streaker by sticking it in some unused corner of the plate. Then you take the now sterile streaker and streak from one line from the last set of streaks. You repeat this four… [cont.]
Answered by Jay Jay - Wed Feb 6 22:37:56 2008
explain how procedure in streak plate method would generate single isolated colonies?
Q. explain how procedure in streak plate method would generate single isolated colonies?
Asked by fain - Wed Jul 29 10:21:26 2009 - - 1 Answers - 0 Comments
A. well,.. it goes like this...nnn
Answered by foxy - Wed Jul 29 10:25:04 2009
Q. explain how procedure in streak plate method would generate single isolated colonies?
Asked by fain - Wed Jul 29 10:21:26 2009 - - 1 Answers - 0 Comments
A. well,.. it goes like this...nnn
Answered by foxy - Wed Jul 29 10:25:04 2009
if you are attemtpting to isolate a single colony from a mixed culture from a streak plate, how can you be ...
Q. sure that the colony you chose to isolate is a pure culture? haha! yes! i am the hitler of bacteria!!
Asked by girl_on_the_couch_inak - Tue Jan 22 10:44:05 2008 - - 3 Answers - 0 Comments
A. If you really want to be sure, pick the colony and immediately streak it onto a fresh plate. Then take a single, well-isolated and well-formed colony from that plate. Even if the original colony wasn't pure, individual coloniew from the second plate should be. (You can even go through another round if you have to.)
Answered by qetzal - Tue Jan 22 19:24:19 2008
Q. sure that the colony you chose to isolate is a pure culture? haha! yes! i am the hitler of bacteria!!
Asked by girl_on_the_couch_inak - Tue Jan 22 10:44:05 2008 - - 3 Answers - 0 Comments
A. If you really want to be sure, pick the colony and immediately streak it onto a fresh plate. Then take a single, well-isolated and well-formed colony from that plate. Even if the original colony wasn't pure, individual coloniew from the second plate should be. (You can even go through another round if you have to.)
Answered by qetzal - Tue Jan 22 19:24:19 2008
Will the isolated colonies always be in the fourth sector on a streak plate?
Q. Will the isolated colonies always be in the fourth sector on a streak plate?
Asked by Me! - Mon Feb 19 00:00:58 2007 - - 2 Answers - 0 Comments
A. I assume you mean streaking a plate in 4 sections with the first being the heaviest (for example if you pick a primary culture and drop a large amount in the first section and streak around the plate) If that is what you mean then no, not always. You dilute the bugs around the plate so that you are pretty sure there will be a place where colonies are sparse enough. So while the most dilute area will probably have isolated colonies, other areas may too. (I do hope that is what you are talking about though!)
Answered by St. Judy's comet - Mon Feb 19 00:10:08 2007
Q. Will the isolated colonies always be in the fourth sector on a streak plate?
Asked by Me! - Mon Feb 19 00:00:58 2007 - - 2 Answers - 0 Comments
A. I assume you mean streaking a plate in 4 sections with the first being the heaviest (for example if you pick a primary culture and drop a large amount in the first section and streak around the plate) If that is what you mean then no, not always. You dilute the bugs around the plate so that you are pretty sure there will be a place where colonies are sparse enough. So while the most dilute area will probably have isolated colonies, other areas may too. (I do hope that is what you are talking about though!)
Answered by St. Judy's comet - Mon Feb 19 00:10:08 2007
How can you determine if the colony that you chose to isolate is a pure culture?
Q. How can you determine if the colony that you chose to isolate is a pure culture?
Asked by Lucy L - Tue Jun 24 11:30:33 2008 - - 3 Answers - 0 Comments
A. Well, I'm sure you know what a pure culture is so I'll delve right into it... First, it must first be grown under aseptic conditions...requiring sterilized instruments and filtered or still air. Isolated colonies are obtained by growth on the suface of a petri dish, you should really use an appropriate growth medium on the dish. I suggest gelling the dish with agar. Now, to isolate a pure culture, the initial sample (inoculum) is manipulated using with an inoculation loop or needle to spread and dilute the cells on the surface of the plate. The objective is to eventually have some areas of the petri dish with isolated single cells. The culture is incubated under appropriate environmental conditions until the cells have grown and… [cont.]
Answered by Lencia S-PJJ(is bunking Y!A-NY!) - Tue Jun 24 11:46:51 2008
Q. How can you determine if the colony that you chose to isolate is a pure culture?
Asked by Lucy L - Tue Jun 24 11:30:33 2008 - - 3 Answers - 0 Comments
A. Well, I'm sure you know what a pure culture is so I'll delve right into it... First, it must first be grown under aseptic conditions...requiring sterilized instruments and filtered or still air. Isolated colonies are obtained by growth on the suface of a petri dish, you should really use an appropriate growth medium on the dish. I suggest gelling the dish with agar. Now, to isolate a pure culture, the initial sample (inoculum) is manipulated using with an inoculation loop or needle to spread and dilute the cells on the surface of the plate. The objective is to eventually have some areas of the petri dish with isolated single cells. The culture is incubated under appropriate environmental conditions until the cells have grown and… [cont.]
Answered by Lencia S-PJJ(is bunking Y!A-NY!) - Tue Jun 24 11:46:51 2008
why do we have to obtain isolated colonies on agar media?
Q. why do we have to obtain isolated colonies on agar media?
Asked by ila - Mon Mar 17 04:00:01 2008 - - 2 Answers - 0 Comments
A. To be able to count the number of bacteria that were plated. Each original bacterium is responsible for a whole colony. If the colonies run into each other, their boundaries become fused and they become hard or impossible to count. We don't always know how densely the bacteria are present in the sample, so it is customary to plate it in a few dilutions in the hope of getting one that is neither too tightly or too sparesely packed with colonies.
Answered by Yaybob - Mon Mar 17 04:03:35 2008
Q. why do we have to obtain isolated colonies on agar media?
Asked by ila - Mon Mar 17 04:00:01 2008 - - 2 Answers - 0 Comments
A. To be able to count the number of bacteria that were plated. Each original bacterium is responsible for a whole colony. If the colonies run into each other, their boundaries become fused and they become hard or impossible to count. We don't always know how densely the bacteria are present in the sample, so it is customary to plate it in a few dilutions in the hope of getting one that is neither too tightly or too sparesely packed with colonies.
Answered by Yaybob - Mon Mar 17 04:03:35 2008
Will isolated colonies always be found in the last section of the streak plate? Explain why or why not.?
Q. Will isolated colonies always be found in the last section of the streak plate? Explain why or why not.?
Asked by Laura A - Wed Oct 29 00:21:57 2008 - - 1 Answers - 0 Comments
A. Not always but they can be. It depends on how well you streaked the microorganism. It is not important whether or not the isolated colonies are in the last section or not of your agar plate - only that you DO have isolated colonies and no contaminations. Such colonies may be in the any of the sections but i find that usually you get nice colonies in the second last or last sections because the earlier portions have a lot of cells (thus, the microorganism has grown in this huge mass).
Answered by Magenta T - Wed Oct 29 01:19:57 2008
Q. Will isolated colonies always be found in the last section of the streak plate? Explain why or why not.?
Asked by Laura A - Wed Oct 29 00:21:57 2008 - - 1 Answers - 0 Comments
A. Not always but they can be. It depends on how well you streaked the microorganism. It is not important whether or not the isolated colonies are in the last section or not of your agar plate - only that you DO have isolated colonies and no contaminations. Such colonies may be in the any of the sections but i find that usually you get nice colonies in the second last or last sections because the earlier portions have a lot of cells (thus, the microorganism has grown in this huge mass).
Answered by Magenta T - Wed Oct 29 01:19:57 2008
Which is the best way to isolate bacteria?
Q. Will I get good isolated colonies if my sample is swapped on the surface on the agar or should I dissolve my sample (biscuit) is some water? Is it possible to get isolated colonies by innoculating the agar plates with a wire loop by wiping the loop on the surface of the food sample which is solid then streak the agar plate with it? Or do I have to prepare the food and make a solution and dip the loop into it. Appreciate any constructive input. Thanks
Asked by Pur R - Wed Mar 21 08:13:40 2007 - - 3 Answers - 0 Comments
A. Hi. When trying to get isolated colonies from food, the sample is usually homogenized and suspended in sterile water. A few dilutions of this solution are made and then plated on agar plates. At least one of these will give you isolated colonies, which is why you do a range of dilutions. The problem with swabbing the food is that you'll more than likely get a lawn of bacteria all over the plate and won't see any isolated colonies. The opposite is true for the loop...you probably won't get enough bacteria to get a good idea of which bacteria (and how many) are really on the biscuit. When you make the water/food solution, you won't use a loop. You'll pipet 100 microliters (more or less depending on how much water you added) of the… [cont.]
Answered by coastgirl1982 - Wed Mar 21 19:30:13 2007
Q. Will I get good isolated colonies if my sample is swapped on the surface on the agar or should I dissolve my sample (biscuit) is some water? Is it possible to get isolated colonies by innoculating the agar plates with a wire loop by wiping the loop on the surface of the food sample which is solid then streak the agar plate with it? Or do I have to prepare the food and make a solution and dip the loop into it. Appreciate any constructive input. Thanks
Asked by Pur R - Wed Mar 21 08:13:40 2007 - - 3 Answers - 0 Comments
A. Hi. When trying to get isolated colonies from food, the sample is usually homogenized and suspended in sterile water. A few dilutions of this solution are made and then plated on agar plates. At least one of these will give you isolated colonies, which is why you do a range of dilutions. The problem with swabbing the food is that you'll more than likely get a lawn of bacteria all over the plate and won't see any isolated colonies. The opposite is true for the loop...you probably won't get enough bacteria to get a good idea of which bacteria (and how many) are really on the biscuit. When you make the water/food solution, you won't use a loop. You'll pipet 100 microliters (more or less depending on how much water you added) of the… [cont.]
Answered by coastgirl1982 - Wed Mar 21 19:30:13 2007
Microbiology: Suppose that you have carefully used aseptic technique to inoculate a sterile BROTH TUBE with?
Q. inoculum from a single isolated colony on a streak plate What microbiological procedure could you perform to verify that your broth subculture was really a PURE culture and not a MIXED culture that contains a contaminant?
Asked by Dalila - Sat Sep 5 08:53:44 2009 - - 1 Answers - 0 Comments
A. a simple question that often tricks new people to micro. you proceed to aseptically re plate the organism from the broth and observe for morphological differences. further tests maybe done depending on what you observe for example gram stain or acid fast stain
Answered by paul - Sat Sep 5 10:11:25 2009
Q. inoculum from a single isolated colony on a streak plate What microbiological procedure could you perform to verify that your broth subculture was really a PURE culture and not a MIXED culture that contains a contaminant?
Asked by Dalila - Sat Sep 5 08:53:44 2009 - - 1 Answers - 0 Comments
A. a simple question that often tricks new people to micro. you proceed to aseptically re plate the organism from the broth and observe for morphological differences. further tests maybe done depending on what you observe for example gram stain or acid fast stain
Answered by paul - Sat Sep 5 10:11:25 2009
is it possible to determine the safety of water by counting the bacteria colonies present in it?
Q. I used agar solution to isolate the bacteria colonies. Will counting each and every colony determine the safety of water???
Asked by chocolateorcheese - Fri Nov 23 21:07:44 2007 - - 4 Answers - 0 Comments
A. Yea- Start counting
Answered by Steve Barb - Fri Nov 23 21:15:09 2007
Q. I used agar solution to isolate the bacteria colonies. Will counting each and every colony determine the safety of water???
Asked by chocolateorcheese - Fri Nov 23 21:07:44 2007 - - 4 Answers - 0 Comments
A. Yea- Start counting
Answered by Steve Barb - Fri Nov 23 21:15:09 2007
what is the purpose of isolating bacterial colonies by streaking method?
Q. what is the purpose of isolating bacterial colonies by streaking method?
Asked by -unknown- - Fri Jun 26 21:46:18 2009 - - 1 Answers - 0 Comments
Q. what is the purpose of isolating bacterial colonies by streaking method?
Asked by -unknown- - Fri Jun 26 21:46:18 2009 - - 1 Answers - 0 Comments
A colony that grow on a starch casein agar turn the agar from yellow to green. Why?
Q. i am isolating actinomycetes from pit soil using starch casein agar. after 5-6 day, yellowish and slimy colonies are formed. It turned the agar from yellow to a dark green colour.
Asked by iluvlife - Mon Jan 15 04:46:53 2007 - - 2 Answers - 1 Comments
A. Could it be that the colony is producing sulphur granules that are reacting with something in the agar?
Answered by Chloe - Mon Jan 15 10:50:55 2007
Q. i am isolating actinomycetes from pit soil using starch casein agar. after 5-6 day, yellowish and slimy colonies are formed. It turned the agar from yellow to a dark green colour.
Asked by iluvlife - Mon Jan 15 04:46:53 2007 - - 2 Answers - 1 Comments
A. Could it be that the colony is producing sulphur granules that are reacting with something in the agar?
Answered by Chloe - Mon Jan 15 10:50:55 2007
Unknown bacteria on streak plate?
Q. Ok I did a streak plate for an unknown bacteria. Where the isolated colonies were on the plate the area was clear, and where the bacteria weren't the area was cloudy. Picture this colonies with clear space around them, then a big area in the middle of the colonies that was just cloudy with no colonies. What bacteria is this?
Asked by robert johnson - Fri Nov 7 23:40:15 2008 - - 2 Answers - 0 Comments
A. You aren't really giving us a lot of information to go one here...I assume we're talking about a blood agar plate, with hemolytic analysis. What you've described is beta hemolysis, and is a hallmark of Strep. pyogenes. Other bacteria can do this too, though, so I can't be sure without more information about the unknown (shape, Gram staining, clusters or strands, etc...).
Answered by BLLYRCKS - Fri Nov 7 23:55:47 2008
Q. Ok I did a streak plate for an unknown bacteria. Where the isolated colonies were on the plate the area was clear, and where the bacteria weren't the area was cloudy. Picture this colonies with clear space around them, then a big area in the middle of the colonies that was just cloudy with no colonies. What bacteria is this?
Asked by robert johnson - Fri Nov 7 23:40:15 2008 - - 2 Answers - 0 Comments
A. You aren't really giving us a lot of information to go one here...I assume we're talking about a blood agar plate, with hemolytic analysis. What you've described is beta hemolysis, and is a hallmark of Strep. pyogenes. Other bacteria can do this too, though, so I can't be sure without more information about the unknown (shape, Gram staining, clusters or strands, etc...).
Answered by BLLYRCKS - Fri Nov 7 23:55:47 2008
Can reptile mites be transfered to mice or rats?
Q. I'm probably going to isolate my hatchlings from their usual room, if they're near my mouse colony, can they affect the mice?
Asked by AFPrisma - Fri Jun 5 03:23:51 2009 - - 2 Answers - 0 Comments
A. Reptile mites are only found on reptiles BUT, they might use the rats or mice as a potential carrier to another host. You can carry the mites to the rest of your collection as well. If your snakes have mites, they should be quarantined away from the rest of your collection until the treatment is done, and you should work with them last, change after working with them and even take a shower. Freezing rodents after euthanasia will kill most parasites like mites so feeding frozen/thawed is another good way to stop the spread of mites that might be hitching a ride on the mice at the time.
Answered by gallianomom2001 - Fri Jun 5 05:11:45 2009
Q. I'm probably going to isolate my hatchlings from their usual room, if they're near my mouse colony, can they affect the mice?
Asked by AFPrisma - Fri Jun 5 03:23:51 2009 - - 2 Answers - 0 Comments
A. Reptile mites are only found on reptiles BUT, they might use the rats or mice as a potential carrier to another host. You can carry the mites to the rest of your collection as well. If your snakes have mites, they should be quarantined away from the rest of your collection until the treatment is done, and you should work with them last, change after working with them and even take a shower. Freezing rodents after euthanasia will kill most parasites like mites so feeding frozen/thawed is another good way to stop the spread of mites that might be hitching a ride on the mice at the time.
Answered by gallianomom2001 - Fri Jun 5 05:11:45 2009
Feeling Isolated.. Plz answer?
Q. Hey ppl... I hav quite a few friends and when they're alone with me, they talk and stuff but when we are in a group they are so lost talking among themselves and dont even care that i am around... And i have to try to get myself into the conversation and they just answer me and get on with their stuff... it kinda makes me feel snubbed...i know they dont do it deliberately but what's wrong with me?? They just dont care even when i am around... and everytime when we are walking and i am in the middle of two of them they kind of stick together and i am pushed back... They dont do it intentionally...but this makes me feel invisible...plz help me... Should i just stop socializing with them??? Am i better off with my mom at a social gathering… [cont.]
Asked by Emily<3Jason - Tue Apr 22 11:22:37 2008 - - 2 Answers - 0 Comments
A. well, everybody does feel isolated sometimes and it takes time to recover from it. i know. but before, what i did when this happened to me was, i gave them what they want which is go away from them and just do my own thing, homeworks and read books. and not long after that, i feel better and i dont really care what people say bout me. just do what you want to do. dont be so desperate to have friends that doesnt know how to appreciate you.
Answered by Leo - Tue Apr 22 11:47:45 2008
Q. Hey ppl... I hav quite a few friends and when they're alone with me, they talk and stuff but when we are in a group they are so lost talking among themselves and dont even care that i am around... And i have to try to get myself into the conversation and they just answer me and get on with their stuff... it kinda makes me feel snubbed...i know they dont do it deliberately but what's wrong with me?? They just dont care even when i am around... and everytime when we are walking and i am in the middle of two of them they kind of stick together and i am pushed back... They dont do it intentionally...but this makes me feel invisible...plz help me... Should i just stop socializing with them??? Am i better off with my mom at a social gathering… [cont.]
Asked by Emily<3Jason - Tue Apr 22 11:22:37 2008 - - 2 Answers - 0 Comments
A. well, everybody does feel isolated sometimes and it takes time to recover from it. i know. but before, what i did when this happened to me was, i gave them what they want which is go away from them and just do my own thing, homeworks and read books. and not long after that, i feel better and i dont really care what people say bout me. just do what you want to do. dont be so desperate to have friends that doesnt know how to appreciate you.
Answered by Leo - Tue Apr 22 11:47:45 2008
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