If you made a streak plate from a pure culture, how many different colony morphologies would you expect to fin?
Q. If you made a streak plate from a pure culture, how many different colony morphologies would you expect to find? 1, 2 , 3 , or 4? anyone? thanks!
Asked by caseychimento - Mon Jan 26 01:25:21 2009 - - 1 Answers - 0 Comments
A. A streak plate from a pure culture (homogeneous strain of bacteria) should produce only one colony morphology. Multiple colony morphologies indicate contamination of some sort.
Answered by WarburgEffect - Mon Jan 26 01:32:19 2009
Q. If you made a streak plate from a pure culture, how many different colony morphologies would you expect to find? 1, 2 , 3 , or 4? anyone? thanks!
Asked by caseychimento - Mon Jan 26 01:25:21 2009 - - 1 Answers - 0 Comments
A. A streak plate from a pure culture (homogeneous strain of bacteria) should produce only one colony morphology. Multiple colony morphologies indicate contamination of some sort.
Answered by WarburgEffect - Mon Jan 26 01:32:19 2009
Why is it necessary to use solid media to obtain a pure culture?
Q. Why is it necessary to use solid media to obtain a pure culture?
Asked by Bob B - Sun Jun 15 00:44:30 2008 - - 1 Answers - 0 Comments
Q. Why is it necessary to use solid media to obtain a pure culture?
Asked by Bob B - Sun Jun 15 00:44:30 2008 - - 1 Answers - 0 Comments
Why is it important to utilize aseptic technique in pure culture?
Q. Why is it important to utilize aseptic technique in pure culture?
Asked by Apple Yoraidyl Marie Y - Sat Dec 6 09:34:42 2008 - - 3 Answers - 0 Comments
A. if u do not utilize aseptic technique you may carry some microbes to the pure culture making it impure. the reason for a pure culture is that the colony is as a result of one bacterium if you introduce another bacterium into the culture it is no longer pure
Answered by @YD@y - Sat Dec 6 09:44:55 2008
Q. Why is it important to utilize aseptic technique in pure culture?
Asked by Apple Yoraidyl Marie Y - Sat Dec 6 09:34:42 2008 - - 3 Answers - 0 Comments
A. if u do not utilize aseptic technique you may carry some microbes to the pure culture making it impure. the reason for a pure culture is that the colony is as a result of one bacterium if you introduce another bacterium into the culture it is no longer pure
Answered by @YD@y - Sat Dec 6 09:44:55 2008
suppose you performed a Gram stain on a sample from a pure culture of bacteria and observed a field of red?
Q. cocci. Adjacent cells were not always the same color. What do you conclude?
Asked by apple1985 - Tue Feb 19 01:33:17 2008 - - 2 Answers - 0 Comments
A. Gram-negative cocci.
Answered by OKIM IM - Tue Feb 19 01:39:48 2008
Q. cocci. Adjacent cells were not always the same color. What do you conclude?
Asked by apple1985 - Tue Feb 19 01:33:17 2008 - - 2 Answers - 0 Comments
A. Gram-negative cocci.
Answered by OKIM IM - Tue Feb 19 01:39:48 2008
1b) Why the 3 pure cultures were much less efficient in degrading crude oil compared to mixed culture?
Q. From a total of 10 pure cultures of different species of bacteria isolated, 3 species of bacteria were found to be able to independently degrade crude oil in soil. However each of these 3 pure cultures could only reduce the oil contamination from 3000ppm to 2900ppm.
Asked by sherry - Sun Aug 5 18:50:48 2007 - - 1 Answers - 0 Comments
A. Bioremediation is is the complicated interaction of microorganisms that can degrade contaminates in soil, water, and the general environment. Many bacteria have been found that can degrade various contaminates - oil, poly-aromatic hydrocarbons (PAOHs), pesticides, etc -- but many do not function well when in pure culture. Why? The chemical they are degrading is a contaminate, thus 'bad'. The microorganism can take the first step of eliminating the 'bad' part of the contaminate. For instance, some types of Gram (-) bacteria can break the aromatic ring in PAOHs. this renders the molecule 'non-toxic'. However, the molecule in a modified form is still present -- the microorganism can not digest the remaining pieces. After awhile, the… [cont.]
Answered by Hecate109 - Mon Aug 6 08:50:56 2007
Q. From a total of 10 pure cultures of different species of bacteria isolated, 3 species of bacteria were found to be able to independently degrade crude oil in soil. However each of these 3 pure cultures could only reduce the oil contamination from 3000ppm to 2900ppm.
Asked by sherry - Sun Aug 5 18:50:48 2007 - - 1 Answers - 0 Comments
A. Bioremediation is is the complicated interaction of microorganisms that can degrade contaminates in soil, water, and the general environment. Many bacteria have been found that can degrade various contaminates - oil, poly-aromatic hydrocarbons (PAOHs), pesticides, etc -- but many do not function well when in pure culture. Why? The chemical they are degrading is a contaminate, thus 'bad'. The microorganism can take the first step of eliminating the 'bad' part of the contaminate. For instance, some types of Gram (-) bacteria can break the aromatic ring in PAOHs. this renders the molecule 'non-toxic'. However, the molecule in a modified form is still present -- the microorganism can not digest the remaining pieces. After awhile, the… [cont.]
Answered by Hecate109 - Mon Aug 6 08:50:56 2007
Why is it necessary to use a solid media to generate a pure culture?
Q. Why is it necessary to use a solid media to generate a pure culture?
Asked by hannah - Thu Mar 11 18:01:50 2010 - - 0 Answers - 0 Comments
Q. Why is it necessary to use a solid media to generate a pure culture?
Asked by hannah - Thu Mar 11 18:01:50 2010 - - 0 Answers - 0 Comments
Why we use a well isolated colony for the preparation of a pure culture?
Q. Why we use a well isolated colony for the preparation of a pure culture?
Asked by Pinky - Thu Dec 6 11:43:48 2007 - - 2 Answers - 0 Comments
A. Theoretically, each colony on your dish comes from a single bacteria, and therefore have the exact same genetic makeup. You'd need a "well isolated" colony because you wouldn't want other surrounding colonies to contaminate the colony you want to pick.
Answered by john d - Thu Dec 6 12:10:17 2007
Q. Why we use a well isolated colony for the preparation of a pure culture?
Asked by Pinky - Thu Dec 6 11:43:48 2007 - - 2 Answers - 0 Comments
A. Theoretically, each colony on your dish comes from a single bacteria, and therefore have the exact same genetic makeup. You'd need a "well isolated" colony because you wouldn't want other surrounding colonies to contaminate the colony you want to pick.
Answered by john d - Thu Dec 6 12:10:17 2007
How can you be absolutely sure that a culture is pure?
Q. Using streak plate method and a sample of e coli broth, we made 4 streaks on the solid agar. After incubation, each had a growth with pink throughout it. How can I be absolutely sure they are pure? Are they suppose to be pink?
Asked by jmp1122_06 - Fri Sep 26 23:07:09 2008 - - 3 Answers - 0 Comments
A. As Bio Girl has correctly said, it would be a good idea to Gram-stain your isolate and see if they are Gram-negative, rod-shaped bacteria. Just because they are Gram-negative and rod-shaped isn't conclusive for Eschericia coli but it gives you a good indication. If the bacteria turns out to be cocci-shaped and Gram-positive, you would know that your culture was contaminated. Have fun! =)
Answered by Emily the Scientist - Sat Sep 27 01:42:09 2008
Q. Using streak plate method and a sample of e coli broth, we made 4 streaks on the solid agar. After incubation, each had a growth with pink throughout it. How can I be absolutely sure they are pure? Are they suppose to be pink?
Asked by jmp1122_06 - Fri Sep 26 23:07:09 2008 - - 3 Answers - 0 Comments
A. As Bio Girl has correctly said, it would be a good idea to Gram-stain your isolate and see if they are Gram-negative, rod-shaped bacteria. Just because they are Gram-negative and rod-shaped isn't conclusive for Eschericia coli but it gives you a good indication. If the bacteria turns out to be cocci-shaped and Gram-positive, you would know that your culture was contaminated. Have fun! =)
Answered by Emily the Scientist - Sat Sep 27 01:42:09 2008
Can a pure culture be prepared directly from a mixed- broth or a mixed-agar-slant culture?
Q. Can a pure culture be prepared directly from a mixed- broth or a mixed-agar-slant culture?
Asked by sha - Tue Sep 9 10:10:22 2008 - - 1 Answers - 0 Comments
A. If you have positive growth in broth, you'll need to subculture it to an agar plate, such as BAP, and observe for growth of isolated colonies after appropriate incubation. Select one of these colonies and subculture it to another agar plate and incubate appropriately. This plate should be a pure culture. If you have positive growth growth on an agar slant, you can subculture an isolated colony to an agar plate to get growth of a pure culture.
Answered by majormomma - Tue Sep 9 21:30:56 2008
Q. Can a pure culture be prepared directly from a mixed- broth or a mixed-agar-slant culture?
Asked by sha - Tue Sep 9 10:10:22 2008 - - 1 Answers - 0 Comments
A. If you have positive growth in broth, you'll need to subculture it to an agar plate, such as BAP, and observe for growth of isolated colonies after appropriate incubation. Select one of these colonies and subculture it to another agar plate and incubate appropriately. This plate should be a pure culture. If you have positive growth growth on an agar slant, you can subculture an isolated colony to an agar plate to get growth of a pure culture.
Answered by majormomma - Tue Sep 9 21:30:56 2008
Can a pure culture be prepared from a mixed culture growing on an agar slant?
Q. Can a pure culture be prepared from a mixed culture growing on an agar slant?
Asked by Ryan - Tue Jun 16 18:52:10 2009 - - 2 Answers - 0 Comments
A. Yes. The techniques used is called "streaking out for single colonies". The aim is to use an agar plate which favours the growth of the particular organism you seek (called a "selective medium"). You pick a loopful of mixed biomass from the original slant, disperse it in some diluent like peptone water, and spread some of the resulting liquid suspension in one cormer of the plate. Then flame the loop and draw it through the area where you spread the liquid, making parallel lines across the plate. Flame the loop again and draw cross-hatches across the lines you just made. Repeat the cross-hatching, flaming the loop each time, until the plate is covered with lines. After incubation you should find an area where single isolated colonies exist. [cont.]
Answered by kev_the_chemist - Tue Jun 16 19:04:36 2009
Q. Can a pure culture be prepared from a mixed culture growing on an agar slant?
Asked by Ryan - Tue Jun 16 18:52:10 2009 - - 2 Answers - 0 Comments
A. Yes. The techniques used is called "streaking out for single colonies". The aim is to use an agar plate which favours the growth of the particular organism you seek (called a "selective medium"). You pick a loopful of mixed biomass from the original slant, disperse it in some diluent like peptone water, and spread some of the resulting liquid suspension in one cormer of the plate. Then flame the loop and draw it through the area where you spread the liquid, making parallel lines across the plate. Flame the loop again and draw cross-hatches across the lines you just made. Repeat the cross-hatching, flaming the loop each time, until the plate is covered with lines. After incubation you should find an area where single isolated colonies exist. [cont.]
Answered by kev_the_chemist - Tue Jun 16 19:04:36 2009
T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Q. T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Asked by sweetness - Sun Apr 19 19:20:16 2009 - - 1 Answers - 0 Comments
A. Generally, we work on the assumption that an isolated colony is pure based on the appearance of surrounding colonies. If they are too dense, then do a dilution of the original sample or of a selected colony and streak again. Presumed pure colonies should be separated by half an inch or more. Traditionally, the 'proof' is in the successive reculturing of a colony, seeing that it breeds true, and in microscopic examination, looking for differently shaped bacteria, etc. There are so many biochemical tests that can be performed and so many bacteria that are similar in appearance and biochemistry that none of these methods are foolproof. If you are looking for a conclusive proof, I suppose one could use genome sequencing if it were a known,… [cont.]
Answered by hogwaump - Thu Apr 23 11:29:27 2009
Q. T streak question: How can you make sure that an isolated colony on a streak plate is really a pure culture?
Asked by sweetness - Sun Apr 19 19:20:16 2009 - - 1 Answers - 0 Comments
A. Generally, we work on the assumption that an isolated colony is pure based on the appearance of surrounding colonies. If they are too dense, then do a dilution of the original sample or of a selected colony and streak again. Presumed pure colonies should be separated by half an inch or more. Traditionally, the 'proof' is in the successive reculturing of a colony, seeing that it breeds true, and in microscopic examination, looking for differently shaped bacteria, etc. There are so many biochemical tests that can be performed and so many bacteria that are similar in appearance and biochemistry that none of these methods are foolproof. If you are looking for a conclusive proof, I suppose one could use genome sequencing if it were a known,… [cont.]
Answered by hogwaump - Thu Apr 23 11:29:27 2009
Can a pure culture be prepared directly from a mixed-broth or a mixed-slant culture?
Q. Why?
Asked by Steenskees - Tue Jan 9 22:19:29 2007 - - 3 Answers - 0 Comments
A. One would isolate individual colonies from the mixed culture using an agar plate, where the microbiologist uses a method of spreading the organisms called 'streak-to-isolate'. This allows for individual colonies to be developed from mixed culture. Then plate the individual colony onto new media using streak-to-isolate to ensure purity of colony. This is common procedure, and I have my college students do this as an independent project.
Answered by teachbio - Tue Jan 9 22:42:22 2007
Q. Why?
Asked by Steenskees - Tue Jan 9 22:19:29 2007 - - 3 Answers - 0 Comments
A. One would isolate individual colonies from the mixed culture using an agar plate, where the microbiologist uses a method of spreading the organisms called 'streak-to-isolate'. This allows for individual colonies to be developed from mixed culture. Then plate the individual colony onto new media using streak-to-isolate to ensure purity of colony. This is common procedure, and I have my college students do this as an independent project.
Answered by teachbio - Tue Jan 9 22:42:22 2007
do you think you have pure culture of an organism on the slant and agar plate?
Q. can we absolutely sure by studying its cultural characteristic? can please explain
Asked by Hanis R - Wed Feb 13 15:47:24 2008 - - 2 Answers - 0 Comments
A. You cannot be absolutely sure that you have a pure culture based on the plates. You can observe the colonies to try to determine any obvious dissimilarities (color, borders, how much they are raised, rate of growth, etc) but you cannot be certain based on a visual examination alone. The culture could have been contaminated with another very similar organism during streaking.
Answered by jenny m - Wed Feb 13 16:04:32 2008
Q. can we absolutely sure by studying its cultural characteristic? can please explain
Asked by Hanis R - Wed Feb 13 15:47:24 2008 - - 2 Answers - 0 Comments
A. You cannot be absolutely sure that you have a pure culture based on the plates. You can observe the colonies to try to determine any obvious dissimilarities (color, borders, how much they are raised, rate of growth, etc) but you cannot be certain based on a visual examination alone. The culture could have been contaminated with another very similar organism during streaking.
Answered by jenny m - Wed Feb 13 16:04:32 2008
Why is aseptic technique necessary for the successful cultivation of pure cultures in the laboratory?
Q. a. to prevent contamination of your cultures b. to allow you to feel confident that if you use a pure culture for an experiment, the results generated will be from only that microorganism c. to prevent contamination of yourself and the bench area d. Aseptic technique is not really necessary. *more than one answer is possible
Asked by Bia S - Wed Jan 27 19:10:57 2010 - - 1 Answers - 0 Comments
A. A,B,C
Answered by Alex - Wed Jan 27 19:18:53 2010
Q. a. to prevent contamination of your cultures b. to allow you to feel confident that if you use a pure culture for an experiment, the results generated will be from only that microorganism c. to prevent contamination of yourself and the bench area d. Aseptic technique is not really necessary. *more than one answer is possible
Asked by Bia S - Wed Jan 27 19:10:57 2010 - - 1 Answers - 0 Comments
A. A,B,C
Answered by Alex - Wed Jan 27 19:18:53 2010
Can a pure culture be prepared directly from a broth or an agar slant mixed culture? Explain.?
Q. Can a pure culture be prepared directly from a broth or an agar slant mixed culture? Explain.?
Asked by amery - Mon Nov 24 00:41:58 2008 - - 2 Answers - 0 Comments
A. Not confidently. A broth culture cannot be presumed as a pure culture unless innoculated from an original pure culture and a mixed culture slant is very difficult to use to isolate colonies. A pure culture must be grown from an innoculation with cells from a SINGLE colony only. Therefore colonies that are very small or very close together introduce the risk of contamination during innocluation.
Answered by azaylia - Mon Nov 24 01:13:34 2008
Q. Can a pure culture be prepared directly from a broth or an agar slant mixed culture? Explain.?
Asked by amery - Mon Nov 24 00:41:58 2008 - - 2 Answers - 0 Comments
A. Not confidently. A broth culture cannot be presumed as a pure culture unless innoculated from an original pure culture and a mixed culture slant is very difficult to use to isolate colonies. A pure culture must be grown from an innoculation with cells from a SINGLE colony only. Therefore colonies that are very small or very close together introduce the risk of contamination during innocluation.
Answered by azaylia - Mon Nov 24 01:13:34 2008
how can you be sure if your unknown microorganism is a pure culture?
Q. how can you be sure if your unknown microorganism is a pure culture?
Asked by heymama - Sun Nov 2 11:38:09 2008 - - 2 Answers - 0 Comments
A. Examine your plate. Are the colonies isolated? Look at the colony characteristics, are they all consistant in size, pigmentation, shape, changes in the agar from the bacteria (hemolysis, different colors on differential/selective media, pitting,etc)? This is somewhat subjective however if they are isolated with consistant colony characteristics, you probably have a pure culture. If you have colonies that appear different, subculture.
Answered by bactiman63 - Sun Nov 2 13:45:55 2008
Q. how can you be sure if your unknown microorganism is a pure culture?
Asked by heymama - Sun Nov 2 11:38:09 2008 - - 2 Answers - 0 Comments
A. Examine your plate. Are the colonies isolated? Look at the colony characteristics, are they all consistant in size, pigmentation, shape, changes in the agar from the bacteria (hemolysis, different colors on differential/selective media, pitting,etc)? This is somewhat subjective however if they are isolated with consistant colony characteristics, you probably have a pure culture. If you have colonies that appear different, subculture.
Answered by bactiman63 - Sun Nov 2 13:45:55 2008
can you determine whether a broth culture is pure by visually inspecting it without a microscope?
Q. an agar deep culture? An agar slant culture?
Asked by apple1985 - Sun Feb 17 19:48:37 2008 - - 1 Answers - 0 Comments
A. if you let it sit out it will get cloudy or grow bacteria if its contaminated
Answered by sweett3_14 - Tue Feb 19 00:35:51 2008
Q. an agar deep culture? An agar slant culture?
Asked by apple1985 - Sun Feb 17 19:48:37 2008 - - 1 Answers - 0 Comments
A. if you let it sit out it will get cloudy or grow bacteria if its contaminated
Answered by sweett3_14 - Tue Feb 19 00:35:51 2008
What celtic language and culture is most pure and healthy?
Q. such as usage, rich culture and so on ...
Asked by Cymro i'r Carn - Thu Oct 25 17:48:43 2007 - - 2 Answers - 0 Comments
A. Purely in terms of numbers it is Welsh, by a long way. Although I believe that Irish Gaelic has a very rich heritage as well, even though it is not spoken very much today.
Answered by Rembrandt Q. Einstein - Thu Oct 25 18:18:30 2007
Q. such as usage, rich culture and so on ...
Asked by Cymro i'r Carn - Thu Oct 25 17:48:43 2007 - - 2 Answers - 0 Comments
A. Purely in terms of numbers it is Welsh, by a long way. Although I believe that Irish Gaelic has a very rich heritage as well, even though it is not spoken very much today.
Answered by Rembrandt Q. Einstein - Thu Oct 25 18:18:30 2007
A pathogenic agent that can neither be filtered from an extract nor grown in pure culture is probably a:?
Q. a. virus b. bacterium c. protist d. cyanobacterium
Asked by singingrocks! - Tue Jun 3 14:03:49 2008 - - 3 Answers - 0 Comments
A. virus pure culture meaning ... it would just be virus I am assuming. Viruses require cells to infect and then produce more virus.
Answered by unknown - Tue Jun 3 14:08:21 2008
Q. a. virus b. bacterium c. protist d. cyanobacterium
Asked by singingrocks! - Tue Jun 3 14:03:49 2008 - - 3 Answers - 0 Comments
A. virus pure culture meaning ... it would just be virus I am assuming. Viruses require cells to infect and then produce more virus.
Answered by unknown - Tue Jun 3 14:08:21 2008
at what temperature does the agar dissolve?at what temperature does it solidify?(PURE CULTURE METHOD IN MICRO?
Q. at what temperature does the agar dissolve?at what temperature does it solidify?(Pure culture method in micro?
Asked by aton P - Sat Sep 5 09:58:42 2009 - - 1 Answers - 0 Comments
A. dissolves at roughly 80oC and solidifies at roughly 35oC.
Answered by stormalive_32722 - Sat Sep 5 12:02:39 2009
Q. at what temperature does the agar dissolve?at what temperature does it solidify?(Pure culture method in micro?
Asked by aton P - Sat Sep 5 09:58:42 2009 - - 1 Answers - 0 Comments
A. dissolves at roughly 80oC and solidifies at roughly 35oC.
Answered by stormalive_32722 - Sat Sep 5 12:02:39 2009
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If you haven't heard of . Pure. Pwnage by now, it's a fabulous gaming show shot in "mockumentary" style, making fun of (and having fun with) gaming . culture. . Though its creators have long said the show would "always be theirs" it appears ...
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